UWEC CERCA 2025

Schistosomiasis is a tropical disease caused by human-infective parasites of the genus Schistosoma. The larval stage, called miracidia, infects a snail intermediate host and matures into cercariae that are shed into water. Cercariae penetrate human skin and migrate to blood vessels connecting the intestines and liver. Here, mature parasites lay eggs that follow the flow of blood into the liver or against the flow into the intestines. Intestinal eggs will mature, be defecated into fresh water, and hatch to miracidia to continue the cycle of infection. In contrast, liver eggs have reached a dead end, but these are routinely harvested from rodent lab hosts for research use and maintenance of the life cycle. Previous evaluation of transcriptomic differences between the liver and intestine eggs revealed distinct functional differences, yet nothing is known about differences between miracidia derived from these eggs. We isolated distinct infected mice tissues and hatched miracidia. Using these miracidia, we performed RNA-seq analysis and found distinct transcriptomic clusters and differentially expressed genes between the samples. Future directions include experiments to test for differences between these miracidia in behavior and infection capacity. These findings will lead to deeper insights about relevant differences between miracidia from different tissues.