We will evaluate the cytotoxic effects of ginger oil on cultured MCF-7 human breast cancer cells. Essential oils, commonly used through topical application or aromatherapy, have been explored for their potential to alleviate the adverse effects of cancer and its treatments, such as chemotherapy. Previous research has demonstrated that ginger oil can trigger cell death in cervical cancer cells within hours in vitro. In our study, MCF-7 cells will be exposed to varying concentrations of ginger oil for two hours, and cytotoxicity will be assessed by measuring lactate dehydrogenase (LDH) levels in the culture medium We hypothesize that ginger oil will exhibit dose-dependent cytotoxic effects on breast cancer cells.